Chargaff'd rule is applicable is applicable to single stranded DNA or double stranded DNA?l
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Since single stranded RNA and DNA do not have complementary bases, Chargaff's rule is not applicable to them.
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The application of Chargaff's rule is still under investigation and according to current research, it only applies on double-stranded DNA. It does not apply to organellar genomes (mitochondria and plastids) smaller than ~20-30 kbp, nor does it apply to single stranded DNA (viral) genomes or any type of RNA genome.
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