Chemistry, asked by ankitpatel938, 1 year ago

Chlorform extraction and cell debris in a centrifuge tube

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Answered by subham123456123456

0

A mixture of phenol:chloroform:isoamyl alcohol (25:24:1) is then added to promote the partitioning of lipids and cellular debris into the organic phase, leaving isolated DNA in the aqueous phase. Following centrifugation, the aqueous phase containing the purified DNA can be transferred to a clean tube for analysis.

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