Question

Choose the odd one w.r.t. type of DNA ends obtained after digestion with restriction enzyme.

Choose answer:

Eco RV


Hind II


Eco RI


Sma I

Answer 1

Answer:

1. one odd

Explanation:

becouse it produces blunt end other three produces sticky end.

Answer 2

Eco RV is the type of DNA ends obtained after digestion with a restriction enzyme.

Restriction enzyme:

• A restriction enzyme, restriction endonuclease, or restrictase is an enzyme that cleaves DNA into pieces at or near restriction sites inside molecules.
• Restriction enzymes are a subset of the larger endonuclease enzyme family.
• A restriction enzyme is a bacterial protein that cleaves DNA sequences at sequence-specific places, resulting in DNA fragments having a known sequence at each end.
• Certain laboratory processes, such as recombinant DNA technology and genetic engineering, require the use of restriction enzymes.

Sticky ends:

• DNA ends are the qualities of the endpoints of linear DNA molecules, which are classified as "sticky" or "blunt" in molecular biology based on the form of the complementary strands at the terminus.
• In sticky ends, one strand is longer than the other, resulting in unpaired bases on the longer strand.
• When the restriction enzyme cuts at one end of the sequence, between two bases on the same strand, and subsequently cuts on the opposite end of the complementary strand, a sticky end is formed.
• This will result in two ends of DNA with some nucleotides but no complementary bases.

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