Chromosome doubling colchicine application
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Thanks for the question!
It is definitely a very interesting question to solve and do some brainstorming.
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The colchicine-induced chromosomal doubling induces a tetraploidy or 4 haploids when applied to Platanus or Plane trees, such as, London Plane, Platanus kerrii, American sycamore, Western sycamore, Platanus orientalis, Platanus acerifolia and Platanus racemosa. When the pre-soaked or already soaked seed of those species given above were exposed to higher concentrations of colchicine about 0.13-0.16 weight per volume the colchicine-induced seed will eventually deplete or shrink from its original size, and when it is compared to those seeds treated with purified distilled water, primarily at really high level of colchicine, the seed germination required for a seed in normal conditions was delayed and spontaneous. The seeds which were colchicine-treated showed a distinction of short and pre-maturely developed thick cotyledons with low endospermic ratio to perispermic ratio, hypocotyls were bulged up and swollen with some natural abiotic stimuli responses, the seeds were not turgid or field with potential water, but, were broad and can induce some chromosome doubling when applying the same to a ungerminated seed. The doubling will increase percentage wise in increase in colchicine concentrations.
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Hope it helps and solves your query!!
It is definitely a very interesting question to solve and do some brainstorming.
**************************************************
The colchicine-induced chromosomal doubling induces a tetraploidy or 4 haploids when applied to Platanus or Plane trees, such as, London Plane, Platanus kerrii, American sycamore, Western sycamore, Platanus orientalis, Platanus acerifolia and Platanus racemosa. When the pre-soaked or already soaked seed of those species given above were exposed to higher concentrations of colchicine about 0.13-0.16 weight per volume the colchicine-induced seed will eventually deplete or shrink from its original size, and when it is compared to those seeds treated with purified distilled water, primarily at really high level of colchicine, the seed germination required for a seed in normal conditions was delayed and spontaneous. The seeds which were colchicine-treated showed a distinction of short and pre-maturely developed thick cotyledons with low endospermic ratio to perispermic ratio, hypocotyls were bulged up and swollen with some natural abiotic stimuli responses, the seeds were not turgid or field with potential water, but, were broad and can induce some chromosome doubling when applying the same to a ungerminated seed. The doubling will increase percentage wise in increase in colchicine concentrations.
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Hope it helps and solves your query!!
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