Question

citrate test is used to identify organism based in their ability to utilize _______ as carbon source

Answer 1

Determines whether bacteria can utilize citrate as a carbon source. utilization of citrate will produce a blue color.Citrate Test Medium: Simmon's Citrate Agar Slant (Initial pH 6.9) | Substrate: Citrate | Enzyme: Citrase | Indicator/Reagent: Brom Thymol Blue | End-product: Sodium Carbonate (Alkaline/Basic pH) | Positive Reaction: Blue Slant (Growth) | * Negative Reaction: Green Slant (No Growth)Citrate TestCitrate ------> Sodium Carbonate | Citrate is broken down into Sodium Carbonate by Citrase | Used to test if an organism can utilize Citrate as its sole carbon source