Classify the microbes. Add a note on isolation, staining techniques for micro-organisms.
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the state of being separate and alone; the act of separating somebody/something
Step 1: Crystal Violet. primary stain added to the specimen smear. ...
Step 2: Iodine. mordant, makes the dye less soluble so it adheres to cell walls. ...
Step 3: Alcohol. the decolorizer, washes away stain from gram-negative cell walls. ...
Step 4: Safranin. counterstain allows dye adherence to gram-negative cells.
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