Question

Cloning vector has the following properties, except one?
(A) Contain genetic marker for selection
(B) Ability to replicate
(C) Multiple restriction site for entry of DNA
(D) Contain nonessential DNA to optimize cloning​

Answer 1

Answer:

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Answer 2

(C) Multiple restriction sites for entry of DNA is the incorrect option

Cloning vectors

• A cloning vector refers to the genetic vehicle that carries a foreign gene into an organism to replicate and amplify the DNA fragment that is inserted.

• Different sources of cloning vectors can be used based on the requirements such as plasmid vectors, phagemids or bacteriophages.
• A cloning vector can only have a single restriction site for the ligation of the gene fragment.
• Once the gene is ligated, it utilizes the host's enzymes and machinery to multiply.

Incorrect options

• The selection of the transformants is done by genetic markers.
• They possess the ability to produce replicas.
• They contain filler DNA to optimize the cloning process.