Question

Codon optimization through a two-step gene synthesis leads to a high-level expression of aspergillus niger lip2 gene in pichia pastoris.

Answer 1

Aspergillus niger lipases are important biocatalysts for a broad range of industrial applications. To enhance the expression level of a newly cloned lipase gene of A. niger in Pichia pastoris, we applied codon optimization and synthesized the full length codon-optimized gene by a two-step gene synthesis strategy.

Answer 2

Explanation:

Codon optimization is a process used to improve gene expression and increase the translational efficiency of a gene of interest by accommodating codon bias of the host organism.