Biology, asked by prashantasethi8479, 1 year ago

Colorimetric method for estimation of haemoglobin

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Answered by samsij7samsij
0

The method to be described for the determination of hemoglobin

depends upon the comparison, in a calorimeter, of carbon monoxide

hemoglobin solutions, one of which has a known hemoglobin

content. Hoppe-Seyler (1) was the first to describe carbon

monoxide hemoglobin and to make use of this stable combination

for estimating the hemoglobin content of blood. He

devised a “double pipette” for comparing the unknown carbon

monoxide hemoglobin solution with the standard carbon monoxide

hemoglobin solution, prepared from hemoglobin crystals; but the

method never came into general use, because of the many technical

difficulties involved. Haldane (2) suggested a much simpler

method for comparing carbon monoxide solutions, using the apparatus

employed by Gowers (3) for comparing oxyhemoglobin

solutions with a picro-carmine standard. This apparatus was

later employed by Sahli (4) who prepared an acid hematin

standard by adding dilute hydrochloric acid to blood.

A critical discussion of the various methods in use for the

estimating of hemoglobin is beyond the scope of this paper. As

Haldane (2) has pointed out, artificially colored solutions and

tinted glass present great difficulties in standardization with a

definite strength of hemoglobin solution. With a certain strength

of color solution or tinted glass, it is possible to imitate quite

perfectly the tint of a given hemoglobin solution provided the

quality of light remains the same. Any variation from these

standard conditions, either in quality of light or strength of

hemoglobin in solution leads to serious errors.

Answered by Anonymous
5

Answer:

 colorimetric method for the measurement of glycosylated hemoglobin is ... NicolA rapid simplified method for routine measurement of glycosylated haemoglobin.

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