Compare and contrast the Hot start and Nested PCR.
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Nested polymerase chain reaction (Nested PCR) is a modification of polymerase ... Touchdown polymerase chain reaction · Hot start PCR · Overlap extension polymerase chain
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Hot Start PCR. Unspecific amplification is a problem that can occur during PCR. Most DNA polymerases that are used for PCR, work best at 68 - 72°C.
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