Question

compare the various types of blotting techniques ​

Answer 1

Answer:

three types of blotting methods: Southern blotting, Northern blotting and Western blotting

Southern Blotting

Southern blotting is a technique for detecting specific DNA fragments in a complex mixture. The technique was invented in mid-1970s by Edward Southern. It has been applied to detect Restriction Fragment Length Polymorphism (RFLP) and Variable Number of Tandem Repeat (VNTR) Polymorphism. The latter is the basis of DNA fingerprinting.

Northern Blotting

Northern blotting is used for detecting RNA fragments, instead of DNA fragments. The technique is called "Northern" simply because it is similar to "Southern", not because it was invented by a person named "Northern".

In the Southern blotting, DNA fragments are denatured with alkaline solution. In the Northern blotting, RNA fragments are treated with formaldehyde to ensure linear conformation.

Western Blotting

Western blotting is used to detect a particular protein in a mixture. The probe used is therefore not DNA or RNA, but antibodies. The technique is also called "immunoblotting"

Explanation:

Answer 2

Answer:

Different blotting is used to detect the different types of micro molecules such as southin blotting is used for DNA analysis Western blotting is four protein analysis ,Northern blotting is for RNA analysis and western for carbohydrate detection.

Explanation:

Please Follow Me And Make Me A Brainlist