Question

Competent cells prepared in your lab have a transformation efficiency of 108
cfu/µg of
plasmid DNA. These competent cells (100 µl) were transformed with 10 ng of plasmid DNA
using heat shock method followed by addition of 900 µl of LB medium. 100 µl of transformed
cells were plated on antibiotic containing plates. The number of colonies expected to be on
the plate would be:

Answer 1

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➢Transformation efficiency is the efficiency by which cells can take up extracellular DNA and express genes encoded by it. This is based on the competence of the cells. It can be calculated by dividing the number of successful transformants by the amount of DNA used during a transformation procedure.