Question

Complete digestion and partial digestion by restriction endonuclease

Answer 1

Answer:

When not all DNA molecules are fully cut by the restriction enzyme(s), some cutting sites in some of the molecules stay intact. This may result in more DNA fragments than expected. Such incomplete (or: "partial") digestion can occur for many reasons: bad quality or an insufficient amount of restriction enzyme.

Answer 2

A partial restriction digest involves performing an incomplete digestion of the plasmid DNA so that, in our example where you have two restriction sites for the enzyme in question, you will end up with three digestion products: one cut at both sites, one cut at the site you want and one cut at the site you don't want.