Question

Concentration of nalidixic acid for bacteria vulture

Answer 1

I'm trying to revive glycerol stock of E. coliDH5alpha by streaking on a Nalidixic acid (NA) containing plate. I have prepared fresh nalidixic acid (15ug/mL) but I'm getting mixed reviews on the optimal concentration of NA to be used. Also, some suggest making it in NaOH and others in water. If it is NaOH, then what dilution should be used to dissolve? When I streak the cells in NA containing plate, even after 12 hours colonies are too small to inoculate. When inoculated in LB with NA then also the growth is very less after 14 hours