Question

conclusion of southern blotting
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Answer 1

Explanation:

Results. The hybridization of probe to the specific DNA on the membrane results to a conclusion that the probe is specific to a fragment on the membrane due to the presence of a complementary sequence in the fragment . ... Below is an example of a typical Southern blot result.

Answer 2

Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. It uses hybridization techniques for the identification of the specific nucleic acids and genes. The blotting technique is a tool used in the identification of biomolecules such ad DNA, mRNA and protein during different stages of gene expression. Protein synthesis involves expression of a DNA segment which gets converted to mRNA to produce the respective protein. Molecules such as DNA, RNA and proteins are subjected to biochemistry analysis which are separated using blotting techniques. In the case of a cell, these molecules are present altogether and hence with the help of blotting scientists are able to recognise a specific molecule out of all others. Blotting is performed by allowing a mixture of molecules of interest pass through a block of gel which separates the molecules based on their molecular sizes. The hence processed molecules are required to be hard-pressed against a suitable membrane which will in turn transfer the molecules from the gel onto a suitable membrane (nylon, nitrocellulose or PVDF) via capillary action. After the molecules are transferred to the membran