conduct experiment to test starch please follow me
Answers
Answer:
Starch testing
1) heat a plant leaf in boiling water for 30 seconds (this stops its chemical reactions)
2) heat it in boiling ethanol for a few minutes (this removes most of its colour)
3) wash with water and spread onto a white tile.
4) add iodine solution from a dropping pipette.
I hope it will be helpful to you friend
Objective:
To test the presence of starch in the given food sample.
Apparatus and materials required:
Test tubes, test-tube stand, test-tube holder, spirit lamp, dropper, filter paper, iodine solution, distilled water, and foodstuff (potato, rice, wheat or maize grains).
Theory:
Starch, a complex carbohydrate, is composed of 15%-20% amylose and 80%-85% amylopectin. It is found in different kinds of cereals such as rice, wheat, maize, etc. After reacting with iodine solution starch forms a dark, blue-black compound. The appearance of blue-black colour is due to the presence of amylase in starch.
Procedure:
- Take a few small, freshly cut pieces of potato or a few grains of rice or wheat or maize in a clean test tube.
- Pour 10 mL distilled water into the test tube.
- Now, boil the contents of the test tube for about 5 minutes.
- Allow the test tube to cool.
- Filter the contents of the test tube through a filter paper.
- Test the obtained filtrate for the presence of starch by the following method.
Precautions:
- Use test-tube holder for holding the test tubes and keep the mouth of the test tube away from yourself while heating.
- Use clean test tubes.
- Do not use too much of iodine solution.