Question

Consider that the proteins adsorb on the surface of an implant using a monolayer model. According to the Langmuir adsorption isotherm:

Where θ is the ratio of sites occupied by the molecule and the total sites available for adsorption, K= 2 (mM)-1 and P is the concentration of the molecule. A cytokine is trapped inside a chamber at concentration of 2 mM. If there are 105  total binding sites, how many of them will be occupied at steady state? Assume equilibrium conditions. Express answer as multiples of 104 ​

Answer 1

Answer:

Adsorption (not to be mistaken for absorption) is the accumulation and adhesion of molecules, atoms, ions, or larger particles to a surface, but without surface penetration occurring. The adsorption of larger biomolecules such as proteins is of high physiological relevance, and as such they adsorb with different mechanisms than their molecular or atomic analogs. Some of the major driving forces behind protein adsorption include: surface energy, intermolecular forces, hydrophobicity, and ionic or electrostatic interaction. By knowing how these factors affect protein adsorption, they can then be manipulated by machining, alloying, and other engineering techniques to select for the most optimal performance in biomedical or physiological applications.

Explanation:

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