Question

Consider the following statements.

(i) Origin of replication or on site is a specific sequence of DNA from where replication initiates.

(ii) Selectable markers are
genes which impart unique features to a vector, e.g. antibiotic resistance.

(iii) Cloning or Recognition sites are short stretches of DNA which specific restriction enzymes can identify and bind to. Reconcile the above statements and explain why these features are considered essential to facilitate cloning
into a vector during RDT​

Answer 1

Answer:

1.The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. ... Synthesis of daughter strands starts at discrete sites, termed replication origins, and proceeds in a bidirectional manner until all genomic DNA is replicated.

2.A selectable marker enables selection of the transformed cells. Generally, these markers impart resistance to phototoxic compounds like antibiotics and herbicides. It is a stable dominant gene and is integral part of transformation vector.