Question

Construction of First Artificial Recombinant DNA

Answer 1

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Answer 2

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(i)It was achieved by linking a gene encoding antibiotic resistance with a native plasmid (an autonomously replicating circular extrachromosomal DNA) of Salmonella typhimurium.

(ii) Stanley Cohen and Herbert Boyer accomplished this in 1972.

(iii) They isolated the antibiotic resistance gene by cutting out a piece of DNA from a plasmid.

(iv) The cutting of DNA at specific locations was carried out by molecular scissors, i.e. restriction enzymes.

(v) The cut piece of DNA was then linked to the plasmid DNA with the enzyme DNA ligase. The plasmid DNA acts as vectors to transfer the piece of DNA attached to it.

(vi) When this DNA is transferred into coli, it could replicate using the new host’s DNA polymerase enzyme and make multiple copies.

(vii) This ability to multiply copies of antibiotic resistance gene in E. coli was called cloning of antibiotic resistance gene in E. coli.

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