Construction of genomic and cdna libraries
Answers
Answer:
genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase.[1] Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.[2]
Answer:
The main consideration in theconstruction of genomic or cDNA libraries is, therefore, the nucleic acid starting material. Since the genome of an organism is fixed, chromosomal DNA may be isolated from almost any cell type in order to prepare genomicDNA.