Biology, asked by mrgoodb62, 1 month ago

CRISPR sgRNA contains sequences for target (20nt), Cas9 handle (42nt) and S. pyogenes Terminator (41nt). Is there a need for host specific Terminator?

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Answered by mdramesh786

Answer:

am creating CRISPRi tool for Lactococcus lactis. Do I need to add an extra Lactococcus Terminator sequence at the end of the sgRNA or is the S. pyogenes Terminator enough

Answered by mtaj5499

Answer:

HI MY NAME IS KULSUM AND I AM ALSO IN CLASS 10TH AND I AM A GIRL

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CRISPR sgRNA contains sequences for target (20nt), Cas9 handle (42nt) and S. pyogenes Terminator (41nt). Is there a need for host specific Terminator?​

Answers

CRISPR sgRNA contains sequences for target (20nt), Cas9 handle (42nt) and S. pyogenes Terminator (41nt). Is there a need for host specific Terminator?