Question

Cross reactivity between mouse and human cells + coculture

Answer 1

Answer:

want to setup a co-culture system of mouse macrophages with Schwann cells.

Normally I would prefer to cultivate mouse Macrophages with mouse Schwann cells. However I found in the literature that people used mouse macrophages with rat Schwann cells. We do have a rat schwann cell line and I would use it if possible but I'm afraid  to use a mixed species approach.

Is this a mixed species co-cultivation a suitable model system?

Will mouse cytokines be recognized by rat cells (and vice versa)?

Has anyone experience with mixed species co cultivation?

Is this accepted in the world of science?

Co-Culture

Macrophage

Cell Culture Techniques

FACS

Primary Cell Culture

Macrophage Polarization