Question

Cultural characteristics of spiral shaped bacteria

Answer 1

cultural characteristics of spiral shaped bacteria is to form the third major morphological category of prokaryotes along with the rod-shaped bacilli and round cocci.

Answer 2

Answer:

a Fluorescent diffusible pigments

Water-soluble pigments produced by bacteria diffuse out of the cells into the surrounding medium. Fluorescent pigments are usually water soluble; the best known examples are the fluorescent pigments produced by some Pseudomonas species; these vary in fluorescence from white to blue-green upon excitation with UV light (Palleroni, 1984). Fluorescent pigment production is not limited to pseudomonads; for instance, fluorescent pigments are produced by several freshwater and marine spirilla (Hylemon et al., 1973) and by some Azotobacter species (Kennedy et al., 2005).

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Inoculate a single streak of the organism across a plate of a colourless medium such as King medium B, which contains the following (per litre of distilled water): pancreatic digest of casein USP, 10.0 g; peptic digest of animal tissue USP, 10.0 g; K2HPO4, 1.5 g; MgSO4·7H2O, 1.5 g; and agar, 14.0 g; pH 7.2.

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After incubation at the appropriate temperature and time, remove the covers from the plates and look for a fluorescent zone surrounding the growth with a UV light (below 260 nm).