DAM method.
Which colour is obtained after addition of working reagent to test and sample solution an
boiling for 20 minutes in estimation of urea nitrogen in urine by DAM method and why?
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Explanation:
Principle Urea in an acidic medium condenses with Diacetyl monoxime at 100°C to form a red coloured complex. Intensity of the colour formed is directly proportional to the amount of urea present in the sample. Mix well and keep the test tubes in boiling water (100°C) for 10 minutes.
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