Question

define centrifugation
pls write in long answers

Answer 1

Centrifugation is a technique which involves the application of centrifugal force to separate particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed. This process is used to separate two miscible substances, but also to analyze the hydrodynamicproperties of macromolecules. More-dense components of the mixture migrate away from the axis of the centrifuge, while less-dense components of the mixture migrate towards the axis. Chemists and biologists may increase the effective gravitational force on a test tube so as to more rapidly and completely cause the precipitate (pellet) to gather on the bottom of the tube. The remaining solution (supernatant) may be discarded with a pipette.[citation needed]. Centrifugation of protein solution, for example, allows elimination of impurities into the supernatant.


The rate of centrifugation is specified by the angular velocity usually expressed as revolutions per minute (RPM), or acceleration expressed as g.