Define Chromatography method.
Answers
Answer:
Chromatography, technique for separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream, called the mobile phase, and a contiguous stationary phase.
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Answer:
Step 1: A horizontal line is drawn near one end (about 1.5 cm from the bottom edge) of the paper.
Step 2: The sample needs to be separated is placed as a small drop or line on to the paper using capillary tube. Labelling the drop by a pencil with an alphabet or number help to identify the compound later. In figure above 3 and 4 are the drops labelled. The drops are then soaked on the paper and dried.
Step 3: The paper is then placed into a sealed container with a swallow layer of suitable solvent. The solvent level must be lower than the pencil line or drop on it. The container need to be covered to stop the solvent to evaporate.
Paper chromatography 6
Step 4: The solvent rises up the paper chromatography taking each component of the sample with it. The components travel with the solvent depends on three things:
The polarity of the sample molecule. The non polar components travel faster than the polar component.
The attraction between the sample molecule and the solvent or solvent mixture.
The attraction between the sample and the silica.
Suppose any sample compound mixture contains three colored molecules green, blue and red. According to their polarity, the order of these compounds is green<blue<red. Thus the most non polar green will travel first along with the mobile phase. Then blue and at last most polar compound the red one.
Step 5: When the solvent rises near the end of the paper then the paper should be taken out from sealed container and air dried. The paper with separated bands of components are then observed under UV-light.