Question

define chromatography write it's two applications

Answer 1

Chromatography is a simple technique through which mixtures gets separated into its components. The mixture to be separated is placed in the filter paper, and with the help of the solvents used in it, components get separated. Two applications: Scientists use this chromatography technique for their analysis purposes

Answer 2

Answer:

$\huge \gray\star \: {\underline{ \underline{\sf \gray{ Chromatography: }}}}$

$\leadsto \boxed{\sf{\orange {Definition}}}$

Chromatography is the separation of colored chemicals . It works because some components of mixture are more soluble than others .

$\leadsto \boxed { \sf {\orange {\: Common \: Use: }}}$

This method analyzes the substances in a mixture .

$\leadsto {\boxed {\sf {\orange {Example: }}}}$

Dyes and inks are mixed to make the colours of food , clothes and pens . We can use this method to find if the colour in the ink of a pen is a pure dye or a mixture of a dyes .

$\leadsto {\boxed{ \sf {\orange {Paper \: Chromatography: }}}}$

In paper chromatography special paper is used to separate the coloured components in a mixture . Chromatography only works for soluble dyes , like in food and pen , not the dyes in clothes . The most soluble dyes moves fastily through filter paper than less soluble dyes .

$\leadsto \boxed { \sf {\orange {\: Application: }}}$

$\bf\purple{Pharmaceutical~ sector}$

• To identify and analyze samples for the presence of trace elements or chemicals.

• Separation of compounds based on their molecular weight and element composition.

• Detects the unknown compounds and purity of mixture.

• In drug development.

$\bf\red{Chemical ~industry }$

• In testing water samples and also checks air quality.

• HPLC and GC are very much used for detecting various contaminants such as polychlorinated biphenyl (PCBs) in pesticides and oils.

• In various life sciences applications

$\bf\pink{ Food ~Industry }$

• In food spoilage and additive detection

• Determining the nutritional quality of food.

$\bf\green{ Forensic ~Science }$

• In forensic pathology and crime scene testing like analyzing blood and hair samples of crime place.

$\bf\blue{ Molecular~ Biology ~Studies }$

• Various hyphenated techniques in chromatography such as EC-LC-MS are applied in the study of metabolomics and proteomics along with nucleic acid research.

• HPLC is used in Protein Separation like Insulin Purification, Plasma Fractionation, and Enzyme Purification and also in various departments like Fuel Industry, biotechnology, and biochemical processes.

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