define cloning site?
Answers
Answer:
A multiple cloning site, also called a polylinker, is a short segment of DNA which contains many restriction sites - a standard feature of engineered plasmids. Restriction sites within an MCS are typically unique, occurring only once within a given plasmid.
Answer:
CLONING SITES :-
Cloning sites are the recognition sites of the restriction enzymes. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes.
In general, cloning vectors are plasmids that are used primarily to propagate DNA. They replicate in E. coli to high copy numbers and contain a multiple cloning site (also called a polylinker) with restriction sites used for inserting a DNA fragment.
E.g :- Examples of cloning vectors are plasmids, cosmids, bacterial artificial chromosomes (BACs), and yeast artificial chromosomes (YACs).
⚕️ NONEXISTENT ⚕️