Question

define explant and callus​

Answer 1

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In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. ... Over time, progenitor cells migrate out of the tissue onto the surface of the dish.

Plant callus is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells are those cells that cover a plant wound. In biological research and biotechnology callus formation is induced from plant tissue samples (explants) after surface sterilization and plating onto tissue culture medium in vitro (in a closed culture vessel such as a Petri dish). The culture medium is supplemented with plant growth regulators, such as auxin, cytokinin, and gibberellin, to initiate callus formation or somatic embryogenesis. Callus initiation has been described for all major groups of land plants.

Answer 2

Answer:

Callus is a hard formation of tissues , Explant is a formation of a new plant on the parent plant. Function : Callus forms the wound part of the surface . Explant organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal.

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