Question

Define the term ELISA​

Answer 1

Answer:

$\underline \mathscr \purple{Question \: : - }$

Define the term ELISA_??

$\underline \mathscr \purple{Answer \: : - }$

ELISA: ELISA is an extremely sensitive test that is used to detect antibodies (Ab) or specific antigens (Ag). It is carried out in a 96 well microtiter plate. The direct ELISA is a test for the presence of antigen. In this procedure, a known antibody is adsorbed to the inside of the well in a microtiter plate. After rinsing to remove excess antibody, the sample suspected of containing the antigen is added. Next, an enzyme linked Ab that can react with Ag is added. If Ag is put in the well, the enzyme linked Ab binds to it and is retained. The colourless substrate for the enzyme is added. Development of colour indicates the presence of the Ag

In indirect ELISA, Ag is added to the microtiter plate well and the Ag attaches to the walls of the microteter plate. After rinsing, to remove excess Ag the serum suspected of containing the Abs is added. Enzyme linked antibody capable of reacting with the constant region of other Abs is then added, followed by addition of the colourless substrate. Development of colour indicates the presence of Ab being identified.

Answer 2

Answer:

It is related to biochemistry which simply means enzyme-linked immunosorbent assay.