Question

Denaturing protein immunoprecipitation from mammalian cells

Answer 1

Denaturing protein immunoprecipitation from Mammalian cells. ... INTRODUCTIONThis protocol describes a denaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background considerably.