describe an experiment to show different parts of embroidered on divot seed
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Objective:
To identify the different parts of an embryo of a dicot seed (Pea, gram or red kidney bean).
Apparatus and Materials Required:
Permanent slides of dicot embryo showing different stages, and a compound microscope.
Procedure:
1. Observe each permanent slide in sequence from early stages to maturity first under low-power magnification and then under high-power magnification of a compound microscope.
2. Draw diagrams and identify the different parts.
3. Label the different regions of the embryo
Observation:
1. Zygote divides transversely forming a basal cell towards the micropyle and a terminal cell towards the chalaza.
2. The basal cell divides by transverse divisions and finally forms 6-10 celled suspensors.
3. The uppermost cell of suspensor swells up to form a vesicular cell while the lowest cell of the suspensor is called hypophysis. Hypophysis forms part of the radical and root cap.
4. The terminal embryonal cell divides by transverse and vertical divisions and forms 16-celled globular embryo.
5. The globular embryo later becomes heart-shaped due to its differentiation into cotyledons.
6. The embryo and cotyledons become larger and curved.
Result:
The prepared permanent slides show successive stages of the development of dicot embryo. The young dicot embryo is globular but changes to heart-shaped structure at maturity due to differentiation into cotyledons.
Precautions:
1. Focus the slides properly.
2. Study the slides first under low-power magnification and then under high-power magnification of the compound microscope.
3. Draw diagrams of the stages as seen under the microscope.
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