Question

Describe any three vector less methods of introducing rDNA into a competent host cell.

Answer 1

Microinjection, electroporation and gene gun method are the three vector less methods of introducing rDNA into a competent host cell.
1- microinjection - it is the process of introducing foreign dna into host cell by injecting dna directly into the nucleus of cell by using micro needles.
2- electroporation - it is the process by which transient holes are created in the plasma membrane of the host cell to facilitate the entry of foreign DNA
3- gene gun method - it is the technique of bombarding microprojectiles coated with foreign dna with very high velocity into the target cell.

Answer 2

Microinjection, electroporation and gene gun approach are the 3 vectors less approaching of introducing rDNA into a competent host cell.

Explanation:

Microinjection:

• It is the action of introducing alien DNA into host cell by inserting DNA directly into the nucleus of cell by using micro needles.

Electroporation:

• It is the action by which transient holes are builder in the plasma layer of the host cell to facilitate the entry of foreign DNA.

Gene gun method:

• It is the technique of bombarding microprojectiles coated with alien DNA with very high velocity into the target cell.

To know more:

Explain any two methods of vectorless gene transfer

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