describe briefly various method of reproduction in bacteria and also mechanism of transformation
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various methods of reproduction in bacteria is following:
1) budding
2)fragmentation
3)asexual reproduction
4)binary fission
5)by conidia, zoospore, endospore formation
mechanism of transformation
firstly recipient cell uptake free DNA released into the environment
then DNA is released into the environment when another bacterial cell dies
all the bacterias are not capable of taking free DNA from the environment. only competent bacteria can do that. those bacterias which ate able to take free DNA and are able to go transformation are called competent bacteria .
competent bacteria have DNA receptor in the surface of its cell.free DNA which is available tin the vicinity of those bacterias can bind to the DNA receptors.
double standed donor DNA binds to specifc receptor on the surface of competent cell.
only single strand of donor DNA reachs to the interior of the recipient cell, the other strand get degraded by nuclease.
recombination with the recipients homologous DNA occurs.
if the DNA is removed the cell will carry ampicillin gene but if the donor DNA not removed the cell will have ampicillin resistant gene.so in a mixture of cell cell contains the original sequence of DNA.
1) budding
2)fragmentation
3)asexual reproduction
4)binary fission
5)by conidia, zoospore, endospore formation
mechanism of transformation
firstly recipient cell uptake free DNA released into the environment
then DNA is released into the environment when another bacterial cell dies
all the bacterias are not capable of taking free DNA from the environment. only competent bacteria can do that. those bacterias which ate able to take free DNA and are able to go transformation are called competent bacteria .
competent bacteria have DNA receptor in the surface of its cell.free DNA which is available tin the vicinity of those bacterias can bind to the DNA receptors.
double standed donor DNA binds to specifc receptor on the surface of competent cell.
only single strand of donor DNA reachs to the interior of the recipient cell, the other strand get degraded by nuclease.
recombination with the recipients homologous DNA occurs.
if the DNA is removed the cell will carry ampicillin gene but if the donor DNA not removed the cell will have ampicillin resistant gene.so in a mixture of cell cell contains the original sequence of DNA.
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