Describe different techniques for enumeration of bacteria
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The methods of enumeration in bacteria can be divided into four categories. Direct methods involve counting the microbes, while indirect methods involve estimation. Viable methods only count cells that are metabolically active, while total counts include dead and inactive cells.
Direct/Viable
A direct/viable method involves a standard plate count, in which repeated dilutions of a sample are counted to calculate the count in the original sample.
Indirect/Viable
Indirect/viable methods such as MPN (most probable number) involve making a statistical inference about the bacteria count based on patterns of growth.
Direct/Total
The bacteria are counted with the aid of fluorescent stains and dyes, which make the bacteria visible with the aid of a fluorescent microscope.
Indirect/Total
Spectroscopy is a form of indirect/total enumeration, which involves estimating the amount of bacteria based on the amount of light passed through the culture by a spectrophotometer.
The methods of enumeration in bacteria can be divided into four categories. Direct methods involve counting the microbes, while indirect methods involve estimation. Viable methods only count cells that are metabolically active, while total counts include dead and inactive cells.
Direct/Viable
A direct/viable method involves a standard plate count, in which repeated dilutions of a sample are counted to calculate the count in the original sample.
Indirect/Viable
Indirect/viable methods such as MPN (most probable number) involve making a statistical inference about the bacteria count based on patterns of growth.
Direct/Total
The bacteria are counted with the aid of fluorescent stains and dyes, which make the bacteria visible with the aid of a fluorescent microscope.
Indirect/Total
Spectroscopy is a form of indirect/total enumeration, which involves estimating the amount of bacteria based on the amount of light passed through the culture by a spectrophotometer.
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