② Describe diffrent methods of decalcification.
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The most common acids used for decalcification are 5-10% solutions of hydrochloric acid (HCl), nitric acid, and formic acid. These acids can be used alone or in combinations. The following should be considered before implementing an acid decalcifying protocol in the laboratory.
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The most common acids used for decalcification are 5-10% solutions of hydrochloric acid (HCl), nitric acid, and formic acid. These acids can be used alone or in combinations. The following should be considered before implementing an acid decalcifying protocol in the laboratory.
Methods and Protocols for Decalcification of Bone Material
- Fixation: 10% Buffered Neutral Formalin for up to 5 days. All fixed specimens are washed in slowly running tap water for a minimum of 30 minutes. ...
- Solutions: ...
- Procedure: ...
- End-Point of Decalcification: ...
- Chemical Test: ...
- Procedure: ...
- Physical Tests: ...
- References:
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