Question

Describe gene amplification (PCR).

Answer 1

Dear Student,

◆ PCR -

• PCR stands for Polymerase Chain Reaction.
• It's in vitro technique used for amplification of nucleic acids.

Steps in polymerase chain reaction -

• Denaturing stage - 94-95 ℃
• Annealing stage - 50-56 ℃
• Extending stage - 72 ℃

Applications of PCR -

1. Classification of organisms
2. DNA fingerprinting
3. Genotyping
4. Prenatal diagnosis
5. Genomic cloning
6. Gentic matching

Thanks dear. Hope this helps you...

Answer 2

Answer:

Denaturation of desired DNA into two strands, each acting as a template, for each strand separate set of primer ( two sets of primer) used, with the help of deoxy(ribo)nucleotides and Taq polymerase (DNA polymerase isolated from Thermus aquaticus ), extension of DNA template occurs, resulting in replication of desired DNA (amplification) = ½ × 6

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