Question

Describe in detail the Gene transfer methods employed in eukaryotic gene cloning.

Answer 1

The bacteria uses conjugation to transfer a DNA segment called T-DNA from its plasmid into the plant. ... The genes to be introduced into the plant are cloned into a plant transformation vector that contains the T-DNA region of the plasmid. An alternative method is agroinfiltration.

Answer 2

Answer:

These synthetic complexes either interact with the cell membrane and promote uptake by endocytosis, or fuse with the membrane and deliver the DNA directly into the cytoplasm.