Describe in detail the Gene transfer methods employed in eukaryotic gene cloning.
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The bacteria uses conjugation to transfer a DNA segment called T-DNA from its plasmid into the plant. ... The genes to be introduced into the plant are cloned into a plant transformation vector that contains the T-DNA region of the plasmid. An alternative method is agroinfiltration.
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These synthetic complexes either interact with the cell membrane and promote uptake by endocytosis, or fuse with the membrane and deliver the DNA directly into the cytoplasm.
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