Describe nomenclature of restriction endonuclease & type 2 restriction endonuclease
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The orthodox type II restriction endonuclease is a homodimer of ∼2 × 30 kDa molecular mass, which recognizes a palindromic sequence 4–8 bp in length, and in the presence of Mg2+ cleaves the two strands of the DNA within or immediately adjacent to the recognition site to give a 5′-phosphate and a 3′-OH end.
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Type IIG restriction endonucleases (e.g., RM. Eco57I) do have a single subunit, like classical Type II restriction enzymes, but require the cofactor AdoMet to be active. Type IIM restriction endonucleases, such as DpnI, are able to recognize and cut methylated DNA.
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