Describe polychrome methylene blue staining for frozen section.
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an alkaline solution of methylene blue that undergoes progressive oxidative demethylation with aging (ripening) to produce a mixture of methylene blue, azures, and methylene violet.
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Polychrome methylene blue is a metachromatic stain that produces good differential coloration in tissues and individual cells. ... The time usually required for staining is only a few seconds (4-8). For the past 16 years, specimens submitted for frozen section have been studied by the rapid smear procedure.
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