Describe the basic principle of fixation with its biological importance
Answers
The mechanism of fixation is dependent on the reagent used. Alcohol based fixations dehydrate cells/tissues, causing proteins to denature and precipitate in situ. Paraformaldehyde causes covalent cross-links between molecules, effectively gluing them together into an insoluble meshwork.
The reason cells must be fixed prior to immunostaining is quite simple. You need to permeabilize cells to allow antibodies to access intracellular structures. Without fixation, the structures in cells would fall apart and diffuse away before you had a chance to finish the antibody incubations and wash steps.
Hope this will help you
EXPLANATION:
In the field of biology, fixation is the protection of natural tissues from rot because of autolysis or rottenness. It ends any progressing biochemical responses and may likewise expand the treated tissues' mechanical quality or solidness.
Tissue fixation is a basic advance in the arrangement of histological areas, its expansive target being to protect cells and tissue segments and to do this so as to take into ‘consideration’ the planning of flimsy, recolored segments. This permits the examination of the ‘tissues' structure’, which is dictated by the ‘shapes and sizes’ of such macromolecules (in and around cells) as proteins and nucleic acids.