Question

Describe the culture of tumour cells in animals

Answer 1

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cell culture:- Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment.
Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment.  The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been established.

Couditions of cell culture:-

Culture conditions vary widely for each cell type, but the artificial environment in which the cells are cultured invariably consists of a suitable vessel containing the following:

a substrate or medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals)

growth factors

hormones

gases (O2, CO2)

a  regulated physico-chemical environment (pH, osmotic pressure, temperature)

Most cells are anchorage-dependent and must be cultured while attached to a solid or semi-solid substrate (adherent or monolayer culture), while others can be grown floating in the culture medium (suspension culture).

Tumors cell culture in animal:-

Successful isolation and growth of tumor cells from freshly excised tumors, depends on several factors including receipt of tissue that contains a significant number of malignant cells for culture, the type of tumor and the in vitro manipulation, which may or may not result in a stable cell line.

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