Describe the mechanism of gene regulation with the help of lac Operon concept.
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The lac operon contains three genes: lacZ, lacY, and lacA. These genes are transcribed as a single mRNA, under control of one promoter.
Genes in the lac operon specify proteins that help the cell utilize lactose. lacZencodes an enzyme that splits lactose into monosaccharides (single-unit sugars) that can be fed into glycolysis. Similarly, lacY encodes a membrane-embedded transporter that helps bring lactose into the cell.
In addition to the three genes, the lac operon also contains a number of regulatory DNA sequences. These are regions of DNA to which particular regulatory proteins can bind, controlling transcription of the operon.
Structure of the lac operon. The DNA of the lac operon contains (in order from left to right): CAP binding site, promoter (RNA polymerase binding site), operator (which overlaps with promoter), lacZ gene, lacY gene, and lacA gene. The activator protein CAP, when bound to a molecule called cAMP (discussed later), binds to the CAP binding site and promotes RNA polymerase binding to the promoter. The lac repressor protein binds to the operator and blocks RNA polymerase from binding to the promoter and transcribing the
The promoter is the binding site for RNA polymerase, the enzyme that performs transcription.
The operator is a negative regulatory site bound by the lac repressor protein. The operator overlaps with the promoter, and when the lac repressor is bound, RNA polymerase cannot bind to the promoter and start transcription.
The CAP binding site is a positive regulatory site that is bound by catabolite activator protein (CAP). When CAP is bound to this site, it promotes transcription by helping RNA polymerase bind to the promoter.
Genes in the lac operon specify proteins that help the cell utilize lactose. lacZencodes an enzyme that splits lactose into monosaccharides (single-unit sugars) that can be fed into glycolysis. Similarly, lacY encodes a membrane-embedded transporter that helps bring lactose into the cell.
In addition to the three genes, the lac operon also contains a number of regulatory DNA sequences. These are regions of DNA to which particular regulatory proteins can bind, controlling transcription of the operon.
Structure of the lac operon. The DNA of the lac operon contains (in order from left to right): CAP binding site, promoter (RNA polymerase binding site), operator (which overlaps with promoter), lacZ gene, lacY gene, and lacA gene. The activator protein CAP, when bound to a molecule called cAMP (discussed later), binds to the CAP binding site and promotes RNA polymerase binding to the promoter. The lac repressor protein binds to the operator and blocks RNA polymerase from binding to the promoter and transcribing the
The promoter is the binding site for RNA polymerase, the enzyme that performs transcription.
The operator is a negative regulatory site bound by the lac repressor protein. The operator overlaps with the promoter, and when the lac repressor is bound, RNA polymerase cannot bind to the promoter and start transcription.
The CAP binding site is a positive regulatory site that is bound by catabolite activator protein (CAP). When CAP is bound to this site, it promotes transcription by helping RNA polymerase bind to the promoter.
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