Question

Describe the method of protoplast isolation and fusion​

Answer 1

Answer:

Protoplasts are isolated by two methods, mechanical and enzymatic. Mechanical isolation method of protoplasts is no more practically used. It is only a historical method. This involves cutting of plasmolysed cells (in fact cell walls) with a sharp blade to release protoplasts.

Answer 2

Answer:

Protoplasts are cells which have had their cell wall removed, usually by digestion with enzymes. The isolation and culture media used vary with the species and with the tissue from which the protoplasts were isolated. ... Protoplasts are used in a number of ways for research and for plant improvement.