Describe the methods used to create recombinant DNA Molecules
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Steps of DNA cloning
Cut open the plasmid and "paste" in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).
Insert the plasmid into bacteria. ...
Grow up lots of plasmid-carrying bacteria and use them as "factories" to make the protein.
Explanation:
Recombinant DNA is the method of joining two or more DNA molecules to create a hybrid. The technology is made possible by two types of enzymes, restriction endonucleases and ligase. A restriction endonuclease recognizes a specific sequence of DNA and cuts within, or close to, that sequence.
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