Question

Describe the population density per unit area measured in a unit of bacteria

Answer 1

Explanation:

First thing we need to know is the Dilution Factor, or how much the original sample is diluted:

here Initially 5mL in 45mL = Final Volume / Sample volume = 50/5 = 10.

Then two serial dilutions of 1/100.

Total Dilution Factor = 10 * 100 *100 = 10^5

CFU/mL = cfu/ml = (no. of colonies x dilution factor) / volume of culture plate

= (137 * 10^5)/0.1

=1.37*10^8

So Total colony forming units = 1.37*10^8 CFU/mL

Converting CFU/mL to Log value