Question

describe the practical application of embryo culture in respect to experimental embryology?​

Answer 1

Answer:

Explanation:

for production of haploid plant

Answer 2

Answer:The im­portance and application of embryo cul­ture on the basis of available information can be broadly grouped into three categories:

1. Importance of embryo culture in relation to biological knowledge

2. Applied aspects of embryo culture

Importance of Embryo Culture In Relation to Biological Knowledge:

In vitro embryo culture has been usefully employed to study some very fundamental prob­lems which are difficult to undertake in embryos enclosed within seeds. Experiments with em­bryos separated from the seeds without interfer­ence from accessory tissues have contributed a lot of information’s to our biological knowledge of plant development in the following aspects.

(i) It helps determining the factors that regu­late the growth of the primodial organs of the seedling plant.

(ii) It helps to study the metabolic and bio­chemical aspects of dormancy and germi­nation

(iii) It helps in analysis of the various parame­ters of embryonic growth.

(iv) The culture of pro embryo helps to under­stand the control of differentiation and the nutritional requirements of progressively smaller embryos.

(v) The culture of surgically dissected embryos segments has facilitated understanding the relationship of the different parts of the em­bryo to its final form in culture.

(vi) An embryo undergoes a gradual transition from the dependence of the zygote to the relatively autonomy of the mature embryo. Changes in nutritional requirements of em­bryos at different stages can be demonstrat­ed by embryo culture.

(vii) The role of accessory tissues can be stud­ied by culturing isolated embryos.

In vitro microsurgical experiments with embryos of cassytha filiformis have shown that cotyledon play an important role in the development of seedling (Fig 10.5). It is evident from the data of Rangaswamy and Rangan (1971) that in C. filiformis the growth factor(s) for shoot development resides in the “radicular-halves” of the cotyledon. If the “plumular halves” of both the cotyle­dons are removed the seedling is well developed but if redicular halves are re­moved the plumule does not grow.

Explanation: