describe the prerequisites of invitro culture experiment
Answers
For sterilization of glassware, working tables, nutrient media and plant materials different techniques are applied. Most common methods of sterilization are:
Use of Chemicals
Use of Dry Heat.
Use of Wet heat or Steam
Ultrafiltration
By Ultraviolet Light
Using Antibiotics
also in laboratory these below requirements r must:----
Nutrient Media : Composition and Preparation
Inorganic Minerals
Growth Hormones
Organic Constituents
in short:
For the tissue culture a good laboratory, similar to a microbiological laboratory, is required which must have facilities for: (i) nutrient medium preparation, sterilization, cleaning and storage of supplies, (ii) aseptic condition for working the living materials, (iii) a controlled environmental conditions for growth and development of cultures, (iv) observation and evaluation of culture as hoped, and (v) recording the observations made during the experiment
In vitro (Latin: in glass; often not italicized in English[1][2][3]) studies are conducted using components of an organism that have been isolated from their usual biological surroundings, such as microorganisms, cells, or biological molecules. For example, microorganisms or cells can be studied in artificial culture media, and proteins can be examined in solutions. Colloquially called "test-tube experiments", these studies in biology, medicine, and their subdisciplines are traditionally done in test tubes, flasks, Petri dishes, etc. They now involve the full range of techniques used in molecular biology, such as the omics.
In contrast, studies conducted in living beings (microorganisms, animals, humans, or whole plants) are called in vivo .