Question

describe the prerequisites of invitro culture experiment​

Answer 1

For sterilization of glassware, working tables, nutrient media and plant materials different techniques are applied. Most common methods of sterilization are:

Use of Chemicals

Use of Dry Heat.

Use of Wet heat or Steam

Ultrafiltration

By Ultraviolet Light

Using Antibiotics

also in laboratory these below requirements r must:----

Nutrient Media : Composition and Preparation

Inorganic Minerals

Growth Hormones

Organic Constituents

in short:

For the tissue culture a good laboratory, similar to a microbiological laboratory, is required which must have facilities for: (i) nutrient medium preparation, sterilization, cleaning and storage of supplies, (ii) aseptic condition for working the living materials, (iii) a controlled environmental conditions for growth and development of cultures, (iv) observation and evaluation of culture as hoped, and (v) recording the observations made during the experiment

Answer 2

In vitro (Latin: in glass; often not italicized in English[1][2][3]) studies are conducted using components of an organism that have been isolated from their usual biological surroundings, such as microorganisms, cells, or biological molecules. For example, microorganisms or cells can be studied in artificial culture media, and proteins can be examined in solutions. Colloquially called "test-tube experiments", these studies in biology, medicine, and their subdisciplines are traditionally done in test tubes, flasks, Petri dishes, etc. They now involve the full range of techniques used in molecular biology, such as the omics.

In contrast, studies conducted in living beings (microorganisms, animals, humans, or whole plants) are called in vivo .