Describe the procedure of mounting a plant cell
Answers
Explanation:
1.Take an onion and remove its outermost peel.
2. Now cut a small part from an inner scale leaf with the help of a blade.
3. Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.
4. Keep this peel in a watch glass containing water?
5. Add two drops of safranin stain in the watch glass to stain the peel.
6. Take a clean slide and put a drop of glycerine in the centre of the slide.
7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.
8. Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.
9. Remove any excessive glycerine with a filter paper.
10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope.
Observations:
A large number of rectangular cells are visible. These cells lie close to each other with intercellular spaces between them. These cells are surrounded by distinct cell walls. These cells have a dark stained nucleus and a large vacuole in the centre.
The following steps are followed to prepare a temporary mount of a leaf peel:
Remove a healthy leaf from the potted plant.
Remove a part of the peel from the lower surface of the leaf.
You can do this by folding the leaf over and gently pulling the peel apart using forceps.
Keeps the peel in a watch glass containing water.
Put a few drops of safranin stain in a watch glass.
After 2-3 minutes take out the peel and place it on a clean glass slide
Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.
Remove the excess stain and glycerin with the help of blotting paper.
Observe the slide under the low-power and high-power magnifications of the compound microscope