Question

describe the quantitative measurements of bacterial growth. ​

Answer 1

Answer:

quantitative measurement of the

Answer 2

Answer:

1. Direct Microscopic Count
2. Counting Chamber Method
3. Proportional Count Method
4. Electronic Counter Method

Explanation:

• Direct Microscopic Count: Another name for the Breed Method.
  Within a specified region, a known volume of cell suspension (0.01 ml) is dispersed uniformly across a glass slide (1 sq. cm).
  After that, the smear is fixed, stained, viewed with an oil immersion lens, and the cells are counted.
  Because scanning the entire region is impracticable, it is typical to count the cells in a few small areas.
  The total cell count is calculated by dividing the total number of microscopic fields per 1 sq cm of cell suspension by the total number of microscopic fields.
  The following calculations are used to determine the overall cell count.
  (a) The area of the microscopic field equals r2.
  =3.14x(0.08)2 (Oil immersion lens radius = 0.08 mm.)
  = 0.02 Sq.mm
  
  (b) Smear Area = 1 sq.cm = 100 sq. millimetre.
  The number of microscopic fields is 1000.02 times 5000.
  Total number of cells per square millimetre Equals average number of cells per field multiplied by 5000.

• Counting Chamber Method: Special microscope slides with chambers designed to hold a cell suspension over a properly defined region engraved into the glass are available.
  The Petroff-Hausser chamber, also known as a haemocytometer, is ruled with squares of known area and built in such a way that a film of known depth may be placed between the slide and the cover slip.
  As a result, the amount of liquid overlaying each squire is precisely understood.

• Proportional Count Method: A standard particle suspension, such as plastic beads, with a known number of particles per volume is combined with an equivalent amount of cell suspension.
  
  This mixed suspension is applied to the slide, which is then fixed and stained.
  Each tiny field's particles and cells are then counted.
  
  The number of fields is used to calculate an average count of the particles and the cell. Assume you acquire an average count of 10 particles and 50 cells per field.
  
  If there are 25,000 particles in 1 mL of standard suspension, the number of cells per 1 mL of suspension is:
  
  50/10x25,000 equals 1,25,000 cells/ml.

• Electronic Counter Method: For direct enumeration of cells in suspension, an electronic equipment known as a Coulter counter can be utilised.
  
  The gadget can count thousands of cells correctly in a few of seconds.
  
  However, the suspending fluid must be devoid of inanimate particles (e.g., dust), because tiny particles will score as cells and bigger particles will obstruct the opening through which the cells travel.

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